Biological Techniques

Biological Techniques 1. (a) I. Plasmids are important tools in molecular biology. Plasmids are small circular DNA that has the ability to enter and iterate in bacterial cells and set up be used as vectors to introduce foreign ingredients into bacteria for cloning and sequencing. Any gene must be inserted into an appropriate location of a plasmid to be expressed. The importance of a plasmid is in the rate of cloning and sequencing when the construction of a recombinant DNA molecule occurs. The drive gene fragment is ligated to a plasmid, and becomes recombinant DNA. Then the plasmid can replicate autonomously in an appropriate host organism. II.

The polymerase chain answer (PCR) is the amplification of DNA sequence by repeated cycles of chain seperation and replication. This is a direct method of making copies of a desire DNA sequence, unlike the technique using plasmids. PCR is a run quite like DNA replication. It is still the process of cardinal DNA strands unwinding, replicati...If you want to get a full essay, set up it on our website: Orderessay

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